p8340 resolving gel buffer Search Results


lysis buffer l1  (Thermo Fisher)
98
Thermo Fisher lysis buffer l1
Lysis Buffer L1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p 8340 10x casein solution vector laboratories cat  (Vector Laboratories)
95
Vector Laboratories p 8340 10x casein solution vector laboratories cat
P 8340 10x Casein Solution Vector Laboratories Cat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protease inhibitor p8340  (Millipore)
90
Millipore protease inhibitor p8340
Protease Inhibitor P8340, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoprecipitation buffer  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology immunoprecipitation buffer
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Immunoprecipitation Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blot ice cold lysis buffer  (Thermo Fisher)
99
Thermo Fisher western blot ice cold lysis buffer
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Western Blot Ice Cold Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ripa buffer p8340  (Beijing Solarbio Science)
90
Beijing Solarbio Science ripa buffer p8340
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Ripa Buffer P8340, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protease phosphatase inhibitors  (Millipore)
90
Millipore protease phosphatase inhibitors
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Protease Phosphatase Inhibitors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis buffer  (Valiant Co Ltd)
99
Valiant Co Ltd lysis buffer
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Lysis Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dea buffer  (Millipore)
96
Millipore dea buffer
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Dea Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ripa buffer r0020  (Beijing Solarbio Science)
90
Beijing Solarbio Science ripa buffer r0020
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Ripa Buffer R0020, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protease inhibitors cell signaling 5872s ripa buffer boston biochem  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc protease inhibitors cell signaling 5872s ripa buffer boston biochem
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Protease Inhibitors Cell Signaling 5872s Ripa Buffer Boston Biochem, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis buffer  (Thermo Fisher)
99
Thermo Fisher lysis buffer
FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The <t>immunoprecipitation</t> procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)
Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The immunoprecipitation procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)

Journal: Journal of cellular physiology

Article Title: Molecular characterization of autophagic and apoptotic signaling induced by sorafenib in liver cancer cells.

doi: 10.1002/jcp.26855

Figure Lengend Snippet: FIGURE 8 Effect of knock‐down of Bim, Bak, and Bax on caspase‐3 activity (a) and autophagic markers (p62, Beclin‐1, and LC3II/I) (b), as well as the binding capacity of Beclin‐1 to Bim, Bak, and Bax (c) in sorafenib‐treated HepG2 cells. The expression of Bim, Bak, and Bax was determined to validate si‐Bim, si‐Bak, and si‐Bax treatments (a). The variables were determined at 24 hr after sorafenib (10 µM) administration. Caspase‐3 activity was measured by commercial chemiluminescence‐based assay as described in Material and Methods. The protein expression of autophagy markers and proapoptotic Bcl‐2 family members was assessed by western‐blot analysis. The immunoprecipitation procedure of Beclin‐1 is described in Material and Methods. The immunoprecipitation using unspecific mouse IgG and anti‐Beclin‐1 antibodies were run in parallel. The expression of Bim, Bak, and Bax has also been measured in the cell lysate. Results are expressed as mean ± standard error of the mean, and with the blots are representative of eight independent experiments. *p ≤0.05, **p ≤0.01, and ***p ≤0.001 between control and sorafenib‐treated cells. The groups with different letters (a, b, c, and d) were significantly different (p ≤0.05)

Article Snippet: A volume of the cell lysate (500 μg protein) was mixed with immunoprecipitation buffer (20mM Tris–HCl pH 7.5, 1% Triton X‐100, 150mM NaCl, 10% glycerol, 1mM Na3VO4, 50mM NaF, 2mM EDTA, 1mM PMSF, commercial proteases inhibitor cocktail (P8340, Sigma‐Aldrich), and 2 μg of anti‐Beclin‐1 antibodies (sc‐48341, Santa Cruz Biotechnology Inc.) at 4°C overnight.

Techniques: Knockdown, Activity Assay, Binding Assay, Expressing, Chemiluminescence Immunoassay, Western Blot, Immunoprecipitation, Control